From owner-biophys@hgmp.mrc.ac.uk Tue Sep 12 02:00:00 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id 8B6F017AE3; Tue, 12 Sep 2000 01:59:59 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6024) id 2CDCD17AE0; Tue, 12 Sep 2000 01:59:58 +0100 (BST) Received: from niobium.hgmp.mrc.ac.uk (niobium [193.62.192.41]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 90A38415D4 for ; Tue, 12 Sep 2000 01:59:57 +0100 (BST) Received: (from news@localhost) by niobium.hgmp.mrc.ac.uk (8.9.3+Sun/8.8.8) id BAA27107 for biophys-list@hgmp.mrc.ac.uk; Tue, 12 Sep 2000 01:59:56 +0100 (BST) X-Authentication-Warning: niobium.hgmp.mrc.ac.uk: news set sender to using -f From: "Lars Thomsen" X-Newsgroups: bionet.biophysics Subject: Electrode problems ! Lines: 17 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2615.200 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2615.200 Message-ID: Date: Tue, 12 Sep 2000 00:59:20 GMT X-Complaints-To: abuse@telia.com X-Trace: newsb.telia.net 968720360 212.181.186.82 (Tue, 12 Sep 2000 02:59:20 CEST) Organization: Telia Internet To: biophys@hgmp.mrc.ac.uk Sender: owner-biophys@hgmp.mrc.ac.uk Precedence: bulk I have a structure where I want to insert a small electrode. The preferable electrode is Ag/AgCl (I guess, since everybody doing electrophysiology is using that). However, the amount of current I have to draw will make the AgCl deposit rather big compared to the structure and then I am afraid that the depletion zone will exend to deep into the bulk solution. I thought that I might use a Pt electrode but that changes the pH with several logs (with the current I need and the volume of solution I have). Do you have any suggestions for an electrode suited for small structures (micrometer scale). Cheers, Lars Thomsen .