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To: bionet-drosophila@net.bio.net
From: oro@cmgm.stanford.edu (anthony oro)
Newsgroups: bionet.drosophila
Subject: Problems with p-element vectors made with gateway system?
Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre
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Fly gurus,
        We're wondering if there is any experience using p element
transformation vectors (for example pUAST) made by gateway cloning
(invitrogen).   Is there any difference in expression or mobilization
from a p-element having the 20-odd basepair recombination site
internally in the polylinker?  We are making  p-element transformation
plasmids this way, but would like to know if we are getting ourselves
into trouble.

Thanks,


Tony

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