From owner-cellbiol@net.bio.net Mon Feb 24 22:00:00 1997 Path: biosci!GOLD.TC.UMN.EDU!bhad0001 From: bhad0001@GOLD.TC.UMN.EDU (Kiran Bhadriraju) Newsgroups: bionet.cellbiol Subject: vital dye in the visible range Date: 25 Feb 1997 07:09:09 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Hi! I am in search of a dye to stain living cells which would absorb in the in the visible range. I looking at hepatocytes through an inverted microscope (attached to an atomic force microscope, but that is irrelevent) and they don't show very well at all. The cells are on a glass cover slip stuck to a steel AFM specimen stub. So my idea is to use a dye in conjunction with a white background for the coverslip. Even if the dye doesn't harm cells only for a few hours, and gives a slight increase in contrast, it would work fine. Would coomassie blue work? (pl excuse if this is a repost, i am not sure if my last mail got through). Many thanks - Kiran bhad0001@gold.tc.umn.edu -------------------------------------------------------------------------------- Kiran Bhadriraju campus mail Box-11, UMHC Biomedical Engineering Institute University of Minnesota, Twin Cities Ph: (612) 626-6360 - (care Dr Hansen) -------------------------------------------------------------------------------- .