From owner-biotechniques@net.bio.net Mon Sep 28 23:00:00 1998 Path: biosci!biosci!not-for-mail From: dharmegn@0.0.0.0 (dharmegn) Newsgroups: bionet.journals.letters.biotechniques Subject: Re: Biotech Date: 29 Sep 1998 06:42:30 -0700 Organization: IPB Lines: 5 Sender: daemon@net.bio.net Approved: mmccarthy@BioTechniques.com Distribution: world Message-ID: <6uqo46$n6n@net.bio.net> NNTP-Posting-Host: net.bio.net I think that avoiding the use of antibiotic resistant plasmid will render very difficult the isolation of a single clone (you will probably have a lot of contaminant bacteria .