From owner-drosophila@net.bio.net Fri Jul 22 23:00:00 1994 Path: biosci!agate!kos2mac13.berkeley.edu!user From: genecutl@mendel.berkeley.edu (gc) Newsgroups: bionet.drosophila Subject: Re: Basal promoters Date: Fri, 22 Jul 1994 19:00:29 -0800 Organization: Bilateral Symmetry Lines: 18 Message-ID: References: <94203.100449LVM3@psuvm.psu.edu> NNTP-Posting-Host: kos2mac13.berkeley.edu In article <94203.100449LVM3@psuvm.psu.edu>, Lakshmi Madabusi wrote: > I am interested in Drosophila promoters, other than hsp70, which have >been used as minimal promoters. (The hsp70 minimal promoter is -50 or >-43 to +89 or far ther downstream.) It doesn't matter whether or how >well it has worked. Lots of people have used the distal promoter of the Alcohol Dehydrogenase gene. You just need around 80bp of this to get transcription in Schneider cell transfections. The actin promoter is also commonly used to drive transcription in Drosophila transfections, but I don't know the size of the promoter fragment used. --gc I know you like rock 'n' roll. Have you considered overdosing on heroin, like many of your pop-star favorites? --Life in Hell .