From owner-celegans@net.bio.net Sun Feb 02 22:00:00 1997
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From: baillie@sfu.ca
Newsgroups: bionet.celegans
Subject: Re: Let-60 effector mutants
Date: Mon, 03 Feb 1997 08:21:52 -0800
Organization: Simon Fraser University
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kariya@icluna.kobe-u.ac.jp wrote:
> 
>         We have recently identified C. elegans homologs of RalGDS, AF6,
> Cdc25 and PLCbeta as candidate Let-60 effectors (WBG 14.5.34-35).  We have
> obtained Tc1-insertion mutants for RalGDS, AF6 and PLCbeta.  We are
> frustrated by unsuccessful freezing of these worms.  This may be due to
> accumulated mutations in these worms.  Alternatively, this may be because
> they have Tc1 within Let-60 effector genes (I do not have experience of
> freezing let-60 or lin-45 mutants).
>         I would appreciate it very much if someone could provide us with a
> special recipe for better freezing of MT3126 worms or let-60-related mutant
> worms.
> Ken-ichi Kariya, M.D.,Ph.D.
> Associate Professor
> Department of Physiology II
> Kobe University School of Medicine
> 7-5-1 Kusunoki-cho, Chuo-ku, Kobe 650, Japan
> Tel: 81-78-341-7451  (ext 3232)　FAX: 81-78-341-3837
> E-Mail: kariya@icluna.kobe-u.ac.jpKen-ichi,

Our original let-60 alleles, recovered by Don Moerman in 1978 have
always frozen pretty well.  Similarly the other alleles recovered 
in my lab (all lethal and balanced by nT1) have frozen and thawed
fine.  It may be the other genes that are causing the problem.

Dave Baillie
IMBB
SFU
CA

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