From owner-dros@hgmp.mrc.ac.uk Wed Nov 12 18:02:24 2003 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 402C87D71F for ; Wed, 12 Nov 2003 18:02:24 +0000 (GMT) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id B90797D6FC for ; Wed, 12 Nov 2003 18:02:23 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 5DF847D452; Wed, 12 Nov 2003 18:02:22 +0000 (GMT) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 9A0617D27F for ; Tue, 11 Nov 2003 19:11:45 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 7F70C7D57B; Tue, 11 Nov 2003 19:11:43 +0000 (GMT) To: bionet-drosophila@net.bio.net From: sandra-schulze@uiowa.edu Newsgroups: bionet.drosophila Subject: drivers & responders in muscle tissue Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: <1068577898.3fb1346aedd75@webmail2.its.uiowa.edu> MIME-Version: 1.0 Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 30CA17D4EB for ; Tue, 11 Nov 2003 19:11:42 +0000 (GMT) X-Received: from night.its.uiowa.edu (night.its.uiowa.edu [128.255.56.106]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 682ED7D438 for ; Tue, 11 Nov 2003 19:11:40 +0000 (GMT) X-Received: from localhost (webmail2-maint.its.uiowa.edu [128.255.56.154]) by night.its.uiowa.edu (8.12.10/8.12.9/ns-mx-1.16) with ESMTP id hABJBdA8065632 for ; Tue, 11 Nov 2003 13:11:39 -0600 X-Received: from dhcp80ff791d.dynamic.uiowa.edu (dhcp80ff791d.dynamic.uiowa.edu [128.255.121.29]) by webmail2.its.uiowa.edu (IMP) with HTTP for ; Tue, 11 Nov 2003 13:11:38 -0600 X-To: dros@net.bio.net X-User-Agent: Internet Messaging Program (IMP) 3.2.1 X-Originating-IP: 128.255.121.29 Date: Wed, 12 Nov 2003 18:02:22 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hi there Drosophila community! I have three questions: First of all, I'd like to find GAL4 drivers that are active in adult structures - especially muscle tissue (indirect flight muscle for instance). Secondly I'd like to knock out specific muscle cells/blocks in either larvae or adults, in order to see if a specific muscle destroyer phenocopies mutants I am testing. An apoptosis promoter might do the trick, and some are available from Bloomington, but I have heard/read somewhere that not all apoptosis promoters are equivalent-some seem to have to be used in combination to have any effect, and some effects are not cell autonomous. Clearly i am not an apoptosis expert. Thirdly i have read/heard/imagined that there are muscle drivers that drive in selected subsets of muscles. This seems like a wonderful system, with an in- built control. If anyone out there can provide me with any information on the above, I'd be most grateful. Many Thanks, Sandra Schulze --- .