From owner-diagnostics@net.bio.net Mon Mar 20 22:00:00 1995 Path: biosci!CMU.CHIANGMAI.AC.TH!mdbci001 From: mdbci001@CMU.CHIANGMAI.AC.TH (Prachya Kongtawelert) Newsgroups: bionet.diagnostics Subject: Re: Use of PVP in ELISA Date: 20 Mar 1995 17:16:47 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 36 Sender: daemon@net.bio.net Distribution: world Message-ID: References: NNTP-Posting-Host: net.bio.net As I have recalled, there was a paper concerning about the use of polyvinylpyrrolidone as a blocker in ELISA in Analytical Biochemistry, 1994. ************* Prachya Kongtawelert, Ph.D. Res Lab for Joint Dis., Dept of Biochem., Fac of Med., Chiangmai University, Chiangmai, Thailand 50200 Tel No. 66-53-894188 Fax No. 66-53-894188, 217144 email:mdbci001@chiangmai.ac.th On 20 Mar 1995, Isla Browning wrote: > As suggested by Bioreba we use polyvinylpyrrolidone in extraction and = > > conjugate buffers when carrying out ELISA for detection of virus in > plant sap. I would be grateful if someone could enlighten me as to why = > > it is used . Is its action as a blocker or as an antioxidant or does = > it have > some other function? > > > Isla Browning > SASA > Edinburgh > 8/3/95 > =01=01=01=01 > > > > .