From owner-drosophila@net.bio.net Wed Oct 05 23:00:00 1994 Path: biosci!bcm!cs.utexas.edu!howland.reston.ans.net!spool.mu.edu!vms.csd.mu.edu!5764OTTOJ From: 5764ottoj@vms.csd.mu.edu (Jeff Otto) Newsgroups: bionet.drosophila Subject: Re: Anesthetic? Date: 6 Oct 1994 20:43:23 GMT Organization: Marquette University - Computer Services Lines: 13 Distribution: world Message-ID: <009858B9.762C20BD@vms.csd.mu.edu> References: <01HHYN56CE768WW4VP@INVERMAY.CRI.NZ> Reply-To: 5764ottoj@vms.csd.mu.edu NNTP-Posting-Host: vmse.csd.mu.edu In article <01HHYN56CE768WW4VP@INVERMAY.CRI.NZ>, FidlerA@agresearch.cri.nz ("Fidler, Andrew") writes: > >Can anyone out there suggest an alternative Drosophila anesthetic to diethyl >ether that might be more suitable (i.e. safer) for use in a classroom >setting? Two alternatives come to mind: CO2 Ice - just place your vials on a bucket of ice, and in a few minutes the flies are anethesized - not necessarily good if activation of the heat shock response will interfere with your experiment, but otherwise simple and effective Jeff .