From owner-celegans@net.bio.net Sun Jan 14 22:00:00 1996
Path: biosci!ANU.EDU.AU!anna.somerville
From: anna.somerville@ANU.EDU.AU (anna somerville)
Newsgroups: bionet.celegans
Subject: synchronous cultures of C. elegans
Date: 14 Jan 1996 19:48:06 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
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Sender: daemon@net.bio.net
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Message-ID: <199601150346.OAA28340@anugpo.anu.edu.au>
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Hi,

I have been isolating eggs from gravid adults and inoculating S medium plus
E. coli with the hatched L1's.  My aim is to grow enough material from each
particular stage to prepare mRNA.  The worms develop well etc but I am
having trouble harvesting the various stages and separating them from the
E. coli using sucrose flotation.  Can anyone out there help?


Anna

Division of Biochemistry and Molecular Biology
School of Life Sciences
ANU
Canberra

email: anna.somerville@anu.edu.au



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