From owner-cellbiol@net.bio.net Thu Jan 23 22:00:00 1997
Path: biosci!JEFLIN.TJU.EDU!rose3
From: rose3@JEFLIN.TJU.EDU (Wes Rose)
Newsgroups: bionet.cellbiol
Subject: Peroxidase problems
Date: 24 Jan 1997 06:50:44 -0800
Organization: Cardeza Foundation/Thomas Jefferson University
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Message-ID: <32E8895F.791A@jeflin.tju.edu>
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I'm trying to use kpl's Histomark Black kit (peroxidase) for
immunostaining, but I'm getting high background staining (nuclear), even
in my controls.  After permiabilizing, I'm incubating O/N at 37=B0C in
0.3% H2O2, and blocking for 1 hr at 37=B0C with 3% BSA, prior to primary
Ab addition.  Between steps, I'm washing 4X.  Any suggestions would be
greatly appreciated.

.