From owner-drosophila@net.bio.net Tue Mar 05 22:00:00 1996
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From: gupta@acsu.buffalo.edu (Vijay Gupta)
Newsgroups: bionet.drosophila
Subject: Help : K  currents from Drosophila embryonic neurons
Date: 6 Mar 1996 21:56:15 GMT
Organization: UB
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Message-ID: <4hl1lv$47p@azure.acsu.buffalo.edu>
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NNTP-Posting-User: gupta


Hi ! 

I am recording potassium currents from Drosophila embryonic neurons, and 
have some technical queries. 

Is it easy to go into the whole cell mode of voltage clamp without 
getting any leak currents? I find that the probability of getting a good 
current is very low in this system. I want to know if this generally 
happens while recording potassium currents from this system. 

Can anyone tell me the success rate of recording potassium currents from 
these neurons?

IS observing a high leak common in these cells? 

How long can the currents be recorded from these cells in the whole cell 
mode?

Can anyone send me a good embryonic culture protocol?

IS there a procedure to obtain isolated neurons other than using 
cytochalasin/chemically enriched medium?

Your help will be greatly appreciated.

I am posting this from a friend's account so could you please reply
to me at khanna@acsu.buffalo.edu

Thanks

Bye
Ashish



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