From owner-drosophila@net.bio.net Mon Mar 21 22:00:00 1994 Path: biosci!daresbury!trane.uninett.no!sunic!pipex!howland.reston.ans.net!europa.eng.gtefsd.com!emory!nigel.msen.com!yale.edu!yale!news.wesleyan.edu!eagle.wesleyan.edu!lappel Newsgroups: bionet.drosophila Subject: Looking for single-fly PCR DNA prep Message-ID: <1994Mar21.161721.1@eagle.wesleyan.edu> From: lappel@eagle.wesleyan.edu Date: 21 Mar 94 16:17:21 EST Nntp-Posting-Host: wesleyan.edu Lines: 11 I would like to be able to do PCR from the genomic DNA of single adult Drosophila. Has anyone got a protocol for a single fly DNA prep that works reliably (stress those last two words) for PCR? Thanks in advance, Laurel F. Appel (LAPPEL@EAGLE.WESLEYAN.EDU) Genetics Group Dept. of Biology Wesleyan University Middletown, CT 06459 .