From owner-celegans@net.bio.net Wed Jun 12 23:00:00 1996 Path: biosci!bcm.tmc.edu!pendragon!news.msfc.nasa.gov!newsfeed.internetmci.com!globe.indirect.com!s82.phxslip4.indirect.com!user From: mthompson@asu.edu (M Thompson) Newsgroups: bionet.molbio.proteins,bionet.celegans Subject: Re: help with a biochemistry lab Date: 13 Jun 1996 20:34:26 GMT Organization: Arizona State University Lines: 28 Message-ID: References: NNTP-Posting-Host: s82.phxslip4.indirect.com Xref: biosci bionet.molbio.proteins:8126 bionet.celegans:941 Egg white lysozyme is a good experiment. Raw product is found in the grocery store. Simple homogenization, isoelectric precipitation, ion exchange chromatography, lyophilization and characterization. Substrate is a strain of bacteria. Then just measure activity on a spectrophotometer. Great experiment. In article , wightman@mendel.berkeley.edu wrote: > I was wondering if anyone could provide suggestions for good proteins to > partially purify/purify for an introductory biochemistry lab for > undergraduates. I'd like to be able to give students an experience with > purification by gel filtration, ion exchange, and/or affinity > chromatography. Preferably, the protein should have an activity that can > be assayed easily and cheaply. The starting material should be something > that's clearly recognizable as "life". Unfortunately, the number of steps > has to be very limited, as time does not allow anything particularly > complicated. Also, it has to be doable at room temperature. > > Any ideas, good references, or protocols? > > Thanks- > Bruce Wightman > Department of Molecular and Cell Biology > 401 Barker Hall- Garriga Lab > University of California > Berkeley, CA 94720-3204 .