From owner-biotechniques@net.bio.net Mon Apr 28 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Robyn Puffenbarger Newsgroups: bionet.journals.letters.biotechniques Subject: protoplast buffer and lysis buffer Date: 29 Apr 1997 06:18:14 -0700 Organization: Medical College of Virginia/VCU Lines: 13 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <3365E158.5F4A@gems.vcu.edu> NNTP-Posting-Host: net.bio.net April 29, 1997 Hello, I have the protocol for the "in-well-lysis" procedure, but have lost the recipies for the protoplast buffer and the lysis buffer. This simple technique lets you quickly see the difference in recombinant plasmids without a 'miniprep.' If anyone has these two recipies and can post them to me or to the list, I would appreciate it. Thanks in advance, Robyn Puffenbarger .