From owner-celegans@net.bio.net Mon Oct 26 22:00:00 1998
Path: biosci!biosci!not-for-mail
From: maduro@lifesci.ucsb.edu (Morris Maduro)
Newsgroups: bionet.celegans
Subject: integration of extrachromosomal arrays -- results of poll on using UV
Date: 27 Oct 1998 06:06:26 -0800
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 28
Sender: daemon@net.bio.net
Approved: wmorgan@acs.wooster.edu
Distribution: world
Message-ID: <714k12$4uv@net.bio.net>
NNTP-Posting-Host: net.bio.net

Hello,

I previously posted a query for results of integration screens that use UV
or oligo-mediated integration.  I received many replies, some of which
suggested using the mutagen MMS as an alternative.  I have posted some of
these, and a short compendium of other (hopefully useful) information, at
the following URL:

http://deficiency.lscf.ucsb.edu/int.html

Thanks to all who replied.  If you would like to suggest corrections or
additions to this web page, please let me know.

[As an aside, I was able to integrate 3/3 lines (0.7% frequency) using a
gamma cell at UCLA (thanks to Arnaud Labrousse for the help!).]

Regards,
Morris

Morris Maduro
Department of MCD Biology
Biological Sciences II
UC Santa Barbara
Santa Barbara, CA 93106
lab: (805) 893-8090
fax: (805) 893-2005



.