From owner-drosophila@net.bio.net Fri Jun 07 23:00:00 1996
Path: biosci!fly.erato.jrdc.go.jp!itokei
From: itokei@fly.erato.jrdc.go.jp (ITO, Kei)
Newsgroups: bionet.drosophila
Subject: Re: Hsp70
Date: 8 Jun 1996 08:37:55 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
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>   I'm trying to find a refrence on expression of the hsCasper
>transformation vector. Does anyone know if it is inducible aby heatshock
>during 0-3 hours of embryogenesis? By immunostaining, I don't see
>expression of my transgene at this stage of embryonic developement;
...
>Kathleen Clark

It is common that the heat shock promoter doesn't work before cell 
formation. If you want to induce expression before the blastodermal 
stage (3 hr AEL) you should better look for other promoters, I think.

  ITO, Kei
  ERATO, at Mitsubishi-Kasei Institute of Life Sciences,
  194 Machida-shi, MinamiOoya 11, Tokyo, Japan
  email : itokei@fly.erato.jrdc.go.jp


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