From owner-metabolic-reg@net.bio.net Fri Aug 13 17:05:00 1999 Path: biosci!newshost.lanl.gov!logbridge.uoregon.edu!newsfeed.direct.ca!tor-nx1.netcom.ca!beaker.tor.sfl.net!bunson.tor.sfl.net!not-for-mail From: "Achim Recktenwald, PhD" Newsgroups: bionet.biophysics,bionet.general,bionet.metabolic-reg Subject: Reducing agents for protein chemistry Lines: 33 X-Priority: 3 X-MSMail-Priority: Normal X-Newsreader: Microsoft Outlook Express 5.00.2314.1300 X-MimeOLE: Produced By Microsoft MimeOLE V5.00.2314.1300 Message-ID: Date: Fri, 13 Aug 1999 17:58:44 GMT NNTP-Posting-Host: router.stressgen.com NNTP-Posting-Date: Fri, 13 Aug 1999 13:58:44 EST Xref: biosci bionet.biophysics:5156 bionet.general:33573 bionet.metabolic-reg:2603 Hi everybody, For some time already, I am looking for a paper / book / webpage / or any other source that contains a list of redox potentials for commonly used reducing agents, like: beta-mercaptoethanol (BME), dithiothreitol (DTT), glutathione, cysteine, Tris(2-carboxyethyl)phosphine hydrochloride (TCEP.HCl) and so on. There must be others around, as well. In many references you can find that DTT is about 10fold better than BME. But I found the wording always so similar that I assume the authors are just copying from each other. Does anybody know of a more exact reference, possibly even including more compounds? We have to produce proteins for in vivo experiments and cannot use BME or DTT in final preparations, because of their toxicity. In addition, I would be interested in their relative strength, i.e., their redox potential. Any answer would be appreciated If possible, send me a copy of your answer also by email Thanks, Achim ARecktenwald@StressGen.com .