From owner-dros@hgmp.mrc.ac.uk  Sun Jul 11 17:05:20 2004
Return-Path: <owner-dros@hgmp.mrc.ac.uk>
X-Original-To: dros-outgoing
Received: from localhost (localhost [127.0.0.1])
	by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 484D37D0C8
	for <dros-outgoing>; Sun, 11 Jul 2004 17:05:20 +0100 (BST)
X-Original-To: dros-list@hgmp.mrc.ac.uk
Received: from localhost (localhost [127.0.0.1])
	by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id D7DCC7D112
	for <dros-list@hgmp.mrc.ac.uk>; Sun, 11 Jul 2004 17:05:19 +0100 (BST)
Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020)
	id EBD7F7D0C8; Sun, 11 Jul 2004 17:05:16 +0100 (BST)
X-Original-To: bionet-drosophila@net.bio.net
Received: from localhost (localhost [127.0.0.1])
	by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id CCFF77D120
	for <bionet-drosophila@net.bio.net>; Sun, 11 Jul 2004 10:27:19 +0100 (BST)
Received: from ms-smtp-02-eri0.socal.rr.com (ms-smtp-02-qfe0.socal.rr.com [66.75.162.134])
	by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 86C2C7D0C8
	for <bionet-drosophila@net.bio.net>; Sun, 11 Jul 2004 10:27:13 +0100 (BST)
Received: from [24.94.72.225] (cpe-24-94-72-225.hawaii.rr.com [24.94.72.225])
	by ms-smtp-02-eri0.socal.rr.com (8.12.10/8.12.7) with ESMTP id i6B9R9Xr007482;
	Sun, 11 Jul 2004 02:27:10 -0700 (PDT)
User-Agent: Microsoft-Outlook-Express-Macintosh-Edition/5.02.2022
Subject: Re: bad infection with microsporidia
From: Hampton - edu <hampton@hawaii.edu>
To: slawek <s.bartoszewski@zmbh.uni-heidelberg.de>
Message-ID: <BD162BCD.A8F%hampton@hawaii.edu>
Mime-version: 1.0
Content-type: multipart/alternative;
   boundary="MS_Mac_OE_3172346829_36636353_MIME_Part"
X-Virus-Scanned: Symantec AntiVirus Scan Engine
Newsgroups: bionet.drosophila
Date: Sun, 11 Jul 2004 17:05:16 +0100 (BST)
Sender: owner-dros@hgmp.mrc.ac.uk
Precedence: bulk

> This message is in MIME format. Since your mail reader does not understand
this format, some or all of this message may not be legible.

--MS_Mac_OE_3172346829_36636353_MIME_Part
Content-type: text/plain; charset="US-ASCII"
Content-transfer-encoding: 7bit

Hello slawek:

    Yes, this is a serious parasite of Drosophila lab cultures.  When I was
working on the mainland at St. Lous, Missouri  back in the 1950's we had
infections in a number of species including melanogaster (see Stalker, H. D.
and H.L. Carson 1964 . A very serious parasite of laboratory Drosophila.
Drosophila Information Service 38:96.) This is a second report (the first
note was in DIS 31:170 1959) - ancient history!  If you don't have access to
old issues of DIS, I still have them and can send you xerox copies of these
two papers. There is also a later paper by a scientist at the University of
Illinois (I can't remember his name) who identified the microsporidians for
us  (we had two different species).

      You should start sterilizing right away if you want to stay in
business!

Good luck and greetings from here!

Hampton Carson


-----
Hampton L. Carson, Professor Emeritus
Department of Cell and Molecular Biology
John A. Burns School of Medicine
University of Hawaii at Manoa
Home: 1314 Kalakaua Ave. Apt 1111
Honolulu, HI 96826
Tel: (808) 983-4522; Fax: -4495


From: s.bartoszewski@zmbh.uni-heidelberg.de (slawek)
Organization: BIOSCI/MRC Rosalind Franklin Centre for Genomics Research
Newsgroups: bionet.drosophila
Date: Thu, 08 Jul 2004 15:23:37 +0100 (BST)
To: bionet-drosophila@net.bio.net
Subject: bad infection with microsporidia


Dear Fly People,

We have been having a really bad problem with a strange infection.  All
stages are affected as follows:

1. Larvae have a part of the body milky white and paralysed, after keeping
in Hoyers medium this part does not clear and is full of small round things
(now I know these are spores).
2. In pupae in a bad case the posterior part is twisted, otherwise they just
die.  Sometimes there is a good looking bottle full of larvae and pupae, but
almost no flies come out.
3. Adults live about a week and show a very poor fertility.

I had sent the flies to an agricultural institution and they were diagnosed
with microsporidia.  To my suggestion that two groups of microsporidia are
mentioned in Ashburner, the lady analysing them said that these would be
rather Octosporea and not Nosema.  She also thought that 2 medicines quoted
in Ashburner, Fumagillin and Benomyl, might not be effective.

The data about these pests is very poor, just a tiny chapter in Ashburner
with a few citations, and I failed to find anything useful in Medline.   I
am afraid that nowadays nobody reports these problems.  I am thinking to
outcross males from the infected lines to healthy females, in a few cases it
helped dramatically but I am not quite sure whether the bugs will not come
back.  

I would appreciate any suggestions from people who faced a similar problem
and managed to overcome it.

         Slawek.



Slawek Bartoszewski
ZMBH, Univ. Heidelberg, Germany
E-Mail:  s.bartoszewski@zmbh.uni-heidelberg.de



--MS_Mac_OE_3172346829_36636353_MIME_Part
Content-type: text/html; charset="US-ASCII"
Content-transfer-encoding: quoted-printable

<HTML>
<HEAD>
<TITLE>Re: bad infection with microsporidia</TITLE>
</HEAD>
<BODY>
Hello slawek:<BR>
<BR>
<BLOCKQUOTE> &nbsp;&nbsp;&nbsp;Yes, this is a serious parasite of Drosophil=
a lab cultures. &nbsp;When I was working on the mainland at St. Lous, Missou=
ri &nbsp;back in the 1950's we had infections in a number of species includi=
ng melanogaster (see Stalker, H. D. and H.L. Carson 1964 . A very serious pa=
rasite of laboratory Drosophila. Drosophila Information Service 38:96.) This=
 is a second report (the first note was in DIS 31:170 1959) - ancient histor=
y! &nbsp;If you don't have access to old issues of DIS, I still have them an=
d can send you xerox copies of these two papers. There is also a later paper=
 by a scientist at the University of Illinois (I can't remember his name) wh=
o identified the microsporidians for us &nbsp;(we had two different species)=
. <BR>
<BR>
</BLOCKQUOTE> &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;You should start sterilizing ri=
ght away if you want to stay in business!<BR>
<BR>
Good luck and greetings from here!<BR>
<BR>
Hampton Carson<BR>
<BR>
<BR>
-----<BR>
Hampton L. Carson, Professor Emeritus<BR>
Department of Cell and Molecular Biology<BR>
John A. Burns School of Medicine<BR>
University of Hawaii at Manoa<BR>
Home: 1314 Kalakaua Ave. Apt 1111<BR>
Honolulu, HI 96826<BR>
Tel: (808) 983-4522; Fax: -4495<BR>
<BR>
<BLOCKQUOTE><BR>
<B>From: </B>s.bartoszewski@zmbh.uni-heidelberg.de (slawek)<BR>
<B>Organization: </B>BIOSCI/MRC Rosalind Franklin Centre for Genomics Resea=
rch<BR>
<B>Newsgroups: </B>bionet.drosophila<BR>
<B>Date: </B>Thu, 08 Jul 2004 15:23:37 +0100 (BST)<BR>
<B>To: </B>bionet-drosophila@net.bio.net<BR>
<B>Subject: </B>bad infection with microsporidia<BR>
<BR>
</BLOCKQUOTE><BR>
<BLOCKQUOTE><FONT FACE=3D"Charcoal">Dear Fly People,<BR>
<BR>
We have been having a really bad problem with a strange infection. &nbsp;Al=
l stages are affected as follows:<BR>
<BR>
1. Larvae have a part of the body milky white and paralysed, after keeping =
in Hoyers medium this part does not clear and is full of small round things =
(now I know these are spores).<BR>
2. In pupae in a bad case the posterior part is twisted, otherwise they jus=
t die. &nbsp;Sometimes there is a good looking bottle full of larvae and pup=
ae, but almost no flies come out.<BR>
3. Adults live about a week and show a very poor fertility.<BR>
<BR>
I had sent the flies to an agricultural institution and they were diagnosed=
 with microsporidia. &nbsp;To my suggestion that two groups of microsporidia=
 are mentioned in Ashburner, the lady analysing them said that these would b=
e rather Octosporea and not Nosema. &nbsp;She also thought that 2 medicines =
quoted in Ashburner, Fumagillin and Benomyl, might not be effective.<BR>
<BR>
The data about these pests is very poor, just a tiny chapter in Ashburner w=
ith a few citations, and I failed to find anything useful in Medline. &nbsp;=
&nbsp;I am afraid that nowadays nobody reports these problems. &nbsp;I am th=
inking to outcross males from the infected lines to healthy females, in a fe=
w cases it helped dramatically but I am not quite sure whether the bugs will=
 not come back. &nbsp;<BR>
<BR>
I would appreciate any suggestions from people who faced a similar problem =
and managed to overcome it. &nbsp;<BR>
<BR>
 &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Slawek.<BR>
<BR>
<BR>
<BR>
 Slawek Bartoszewski &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<BR>
 ZMBH, Univ. Heidelberg, Germany &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;=
&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;<BR>
 E-Mail: &nbsp;s.bartoszewski@zmbh.uni-heidelberg.de</FONT> <BR>
</BLOCKQUOTE><BR>
</BODY>
</HTML>


--MS_Mac_OE_3172346829_36636353_MIME_Part--


.