From owner-celegans@net.bio.net Tue Jun 11 23:00:00 1996 Path: biosci!rutgers!csn!nntp-xfer-1.csn.net!torn!nott!cunews!freenet-news.carleton.ca!FreeNet.Carleton.CA!au905 From: au905@FreeNet.Carleton.CA (Guy Tanentzapf) Newsgroups: bionet.celegans Subject: Markers for transgenic worm lines Date: 12 Jun 1996 04:48:56 GMT Organization: The National Capital FreeNet Lines: 17 Sender: au905@freenet.carleton.ca (Guy Tanentzapf) Message-ID: <4pli7o$f3f@freenet-news.carleton.ca> Reply-To: au905@FreeNet.Carleton.CA (Guy Tanentzapf) NNTP-Posting-Host: freenet.carleton.ca It appears to me that the rol-6 (prf4) is the most used coninjected marker when making transgenic worms. However on the bus today i thought maybe if one injected GFP containing plasmid it might be easier to find transgenic worms, which ,ight be usefull especially when injecting in some tricky backgrounds which dont move a lot (like unc's for example). Im sure someone out there had a similar idea and might have tried it... in any case what do you think? Guy, -- "Ah, make the most of what we yet may spend/Before we too into the dust descend" - Omar Khayyam "Rubaiyat" "In science we pretend that we are immortal" - Max Delbruck .