From owner-biotechniques@net.bio.net Sun Jul 20 23:00:00 1997 Path: biosci!biosci!not-for-mail From: Michele Markstein Newsgroups: bionet.journals.letters.biotechniques Subject: Protein Transfection Date: 21 Jul 1997 11:58:39 -0700 Organization: University of Chicago Lines: 16 Sender: daemon@net.bio.net Approved: MMCCARTHY@BIOTECHNET.COM Distribution: world Message-ID: <33CF839B.64B1@midway.uchicago.edu> NNTP-Posting-Host: net.bio.net I would like to transfect yeast cells with proteins, and would appreciate any information regarding protein transfection (in any cell type). Becasue the yeast cell wall may be an obstacle, my first approach will be to fuse liposomes to spheroplasted yeast cells. Therefore, I am also intereseted in general inights/information about yeast lipofection. In case you are wondering why I am transfecting proteins instead of DNA, my project is to study the effects of prions--a protein based phenomenon. Thanks for your time. Sincerely, Michele Markstein .