From owner-drosophila@net.bio.net Sun Oct 02 23:00:00 1994 Path: biosci!galaxy.ucr.edu!ihnp4.ucsd.edu!usc!howland.reston.ans.net!pipex!lyra.csx.cam.ac.uk!drm-mac1.welc.cam.ac.uk!user From: drm21@mole.bio.cam.ac.uk (David Micklem) Newsgroups: bionet.drosophila Subject: Re: myc epitope tag in flies Date: Mon, 03 Oct 1994 13:03:48 +0000 Organization: Wellcome/CRC Institute Lines: 47 Message-ID: References: NNTP-Posting-Host: drm-mac1.welc.cam.ac.uk In article , jh6u@virginia.edu (Jay Hirsh) wrote: > Hello, > > I am interested in "tagging" a protein with an 11 amino acid c-myc epitope > tag to be expressed in flies. I have found a few examples in which proteins > have been transgenically expressed and immunostained using a myc tag > specific monoclonal Ab, however all of these genes were expressed behind a > heat shock promoter. > > Do any of you know of a case in which someone has tagged a gene that is > driven by its endogenous promoter and sucessfully immunostained tissues from > the transgenic flies? If so, could you please e-mail me at: meller@virginia.edu > > Thanks in advance, > > Margaret Meller > Univ. of Virginia > Dept of Biology Hi Margaret, I've made myc-tagged constructs driven by the maternal alpha4 tubulin and bicoid promotors, and have had no problems with detecting the tag in any of them. While we're on the subject, I've nearly run out of the anti-myc 9E10 monoclonal antibody I've been using. I've also heard that the antibody from some suppliers is or was not very good (Im afraid I dont know which supplier). Can anyone recommend a good source for anti-myc antibody that will work in embryos and on western blots? Cheers, David _____________________________________________________________ D.R.Micklem, Wellcome/CRC Institute, Time flies like an arrow... Tennis Court Road, Cambridge CB2 1QR Fruit flies like a banana. UK Tel: [+44] (0)223 334129 Email:drm21@mole.bio.cam.ac.uk Fax: [+44] (0)223 334089 _____________________________________________________________ .