From owner-drosophila@net.bio.net Sun Jul 18 23:00:00 1993 Path: biosci!parc!decwrl!ames!agate!library.ucla.edu!news.mic.ucla.edu!unixg.ubc.ca!acs.ucalgary.ca!sjszarka From: sjszarka@acs.ucalgary.ca (Steven Joseph Szarka) Newsgroups: bionet.drosophila Subject: Heterologous complementation in Drosophila? Keywords: heterologous, complementation, mutant Message-ID: Date: 19 Jul 93 04:52:20 GMT Followup-To: sjszarka@acs.ucalgary.ca Organization: The University of Calgary, Alberta Lines: 24 We do not work with Drosophila, but are interested in complementing some of the Drosophila mutants with a gene from another eukaryotic organism. Is anyone aware of experiments (published or not) whereby stable transformation and expression of a heterologous eukaryotic gene was shown to complement a Drosophila mutant deficient in an equivalent function? We are interested in any suggests from Drosophila researchers which may help us avoid common or previously encountered problems with similar experiments. As an aside: Would most people consider such an experiment (if complementation works) strong enough evidence to assign a function to the heterologous gene? ie. How strict is complementation? Thanks in advance for any advise and comments Steve Szarka University of Calgary sjszarka@acs.ucalgary.ca .