From owner-dros@hgmp.mrc.ac.uk Mon Jan 12 14:15:49 2004 Return-Path: X-Original-To: dros-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 0EF5B7D70A for ; Mon, 12 Jan 2004 14:15:49 +0000 (GMT) X-Original-To: dros-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 77C6A7DB2D for ; Mon, 12 Jan 2004 14:15:48 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id CA8947D70A; Mon, 12 Jan 2004 14:15:47 +0000 (GMT) X-Original-To: bionet-drosophila@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 99D3D7D7DC for ; Thu, 8 Jan 2004 21:41:30 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 25AC67D1CC; Thu, 8 Jan 2004 21:41:29 +0000 (GMT) To: bionet-drosophila@net.bio.net From: jps10@po.cwru.edu (Jayashree Prasad-Sinha) Newsgroups: bionet.drosophila Subject: Immunostaining polytenes Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: MIME-version: 1.0 Content-type: text/plain; charset=US-ASCII Content-transfer-encoding: 7BIT X-Original-To: dros@net.bio.net X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 45D407D7DC for ; Thu, 8 Jan 2004 21:41:28 +0000 (GMT) X-Received: from ims-msg.TIS.CWRU.Edu (northuist.CNS.CWRU.Edu [129.22.104.60]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 1F5967D1CC for ; Thu, 8 Jan 2004 21:41:27 +0000 (GMT) X-Received: from conversion-daemon.ims-msg.cwru.edu by ims-msg.cwru.edu (iPlanet Messaging Server 5.2 HotFix 1.10 (built Jan 23 2003)) id <0HR601601X9V1A@ims-msg.cwru.edu> for dros@net.bio.net; Thu, 08 Jan 2004 16:35:37 -0500 (EST) X-Received: from [129.22.224.248] (tmp00039397e41e.GENE.CWRU.Edu [129.22.224.248]) by ims-msg.cwru.edu (iPlanet Messaging Server 5.2 HotFix 1.10 (built Jan 23 2003)) with ESMTP id <0HR601292XBD5F@ims-msg.cwru.edu> for dros@net.bio.net; Thu, 08 Jan 2004 16:35:37 -0500 (EST) X-To: dros@net.bio.net X-User-Agent: Microsoft-Entourage/10.0.0.1331 Date: Mon, 12 Jan 2004 14:15:47 +0000 (GMT) Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Hello! Recently I have been facing a peculiar problem with immunostaining polytene chromosomes. The stain appears grainy - as if the antibodies are minute grains, instead of the usual bands. Has anyone faced similar difficulty? How did you overcome it? What could be causing it? I use the standard protocol of Paro. Please send any suggestions to my email. I am not a member of bionet. Thank you, Jayashree --- .