From owner-metabolic-reg@net.bio.net Tue Nov 05 22:00:00 1996 Path: biosci!biosci!not-for-mail From: David Stepp Newsgroups: bionet.biology.cardiovascular,bionet.metabolic-reg,bionet.molbio.ageing,bionet.molbio.evolution,bionet.molbio.proteins,bionet.molecules.peptides,bionet.neuroscience Subject: Physiologic Application of Molecular Biology Date: 5 Nov 1996 16:38:08 -0800 Organization: University of Wisconsin, Madison Lines: 24 Sender: biohelp@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net Xref: biosci bionet.biology.cardiovascular:1263 bionet.metabolic-reg:869 bionet.molbio.ageing:3035 bionet.molbio.evolution:5287 bionet.molbio.proteins:9202 bionet.molecules.peptides:494 bionet.neuroscience:16569 Many of us spend our days (and nights!) cloning, expressing and/or knocking out genes that interest us. Often times, however, we are too deep in the trees and lose sight of what our gene's function is in the context of a whole animal or even at the organ level. I would be interested to know what level of enthusiasm there is out there for studying the physiology of your favorite gene. If you are overexpressing a transcription factor that regulates cardiac gene expression, for example, what effect does this have on cardiac performance, blood pressure etc.? Granting agencies are asking for more than molecular biology these days; they want relevance to a physiological or pathophysiological process. Within this context, would you be interested in learning physiology techniques (blood pressure measurements, cardiac and smooth contractility, shear stress, electrophysiology etc) in a 2-3 week course as a means of broadening your knowledge base and enhancing your experimental research?? Please forward your thoughts and comments to jmiano@post.its.mcw.edu. Thanks in advance! Joe Miano & David W. Stepp .