From owner-cellbiol@net.bio.net Wed Jan 22 22:00:00 1997
Path: biosci!rutgers!gatech!EU.net!uknet!usenet1.news.uk.psi.net!uknet!uknet!lyra.csx.cam.ac.uk!cok-mac4.gen.cam.ac.uk!user
From: bdmc@mole.bio.cam.ac.uk (Brian D. McCabe)
Newsgroups: bionet.cellbiol
Subject: Re: Show me the product name for expression of soluble recombinant protein in E.coli
Date: Thu, 23 Jan 1997 03:51:15 +0000
Organization: Dept of Genetics, Univ of Cambridge
Lines: 16
Message-ID: <bdmc-2301970351150001@cok-mac4.gen.cam.ac.uk>
References: <32E3A9B6.2680@ccMail.bms.com> <32E6A594.1BC0@umich.edu>
NNTP-Posting-Host: cok-mac4.gen.cam.ac.uk
X-Newsreader: Value-Added NewsWatcher 2.0b22.0+

In article <32E6A594.1BC0@umich.edu>, ino@umich.edu wrote:

> Hello, Everyone.
> 
> I am expressing mammalian proteins in E. coli with T7 systems.
> Several proteins were not soluble and is assumed to make
> inclusion body.
> 
> So I would like to try the hosts expressing thioredoxin,
> which make proteins more soluble.
> Once I saw it the commercial in some journals.
> I forget the name of the product.

The system you are looking for is the pET-32a-c(+) expression vector which
has a trx tag.  Its made by R&D systems, email: info@rndsystems.co.uk or
try the web at http://www.ingenius-molbiol.com

.