From owner-celegans@net.bio.net Fri Jul 23 17:10:00 1999 Path: biosci!biosci!not-for-mail From: Jaya Bandyopadhyay Newsgroups: bionet.celegans Subject: Rescue Experiments! Date: 23 Jul 1999 11:10:40 -0700 Organization: K-JIST Lines: 37 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <7nab70$epa@net.bio.net> NNTP-Posting-Host: net.bio.net I would appreciate if you help me with some valuable suggestions regarding rescue experiments. I started already with the whole cosmid and later plan to inject fragments of the cosmid to narrow down and assess the boundaries of the rescuing fragments. 1)What concentrations of the cosmid and the rol-6 genes should I inject into the mutant background? 2)What percentage of F1 rollers can I expect? 3) Is it possible to chase rollers from F2 and subsequent generations? I ask this since I did not get any F2 rollers, and for your information my F1 rollers were too less. 4) How many generations is it necessary to study to believe that rescue is really working? I thank once again for your kind cooperation and help. Sincerely, Jaya Jaya Bandyopadhyay Developmental Genetics Laboratory Department Of Life Science Kwang-ju Institute of Science & Technology (K-JIST) 1 Oryong-dong, Puk-Gu Kwangju 500-712, Korea Tel: +82 62 970-2563 (Lab) Email: bjaya@geguri.kjist.ac.kr Content-Type: text/x-vcard; charset=iso-2022-kr; name="vcard.vcf" Content-Transfer-Encoding: 7bit Content-Description: Card for Jaya Bandyopadhyay Content-Disposition: attachment; filename="vcard.vcf" Attachment converted: MorganDuo:vcard.vcf (TEXT/NISI) (0000CF42) .