From owner-celegans@hgmp.mrc.ac.uk Sat May 27 13:29:16 2000 Return-Path: Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 110) id AF94717AD0; Sat, 27 May 2000 13:29:15 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id 9F97417AD6; Sat, 27 May 2000 13:29:14 +0100 (BST) To: celegans@net.bio.net Newsgroups: bionet.celegans Date: Fri, 26 May 2000 21:55:21 +0100 From: jglomset@u.washington.edu ("John A. Glomset") Subject: cooperation needed Organization: University of Washington Message-Id: <20000527122914.9F97417AD6@mercury.hgmp.mrc.ac.uk> Sender: owner-celegans@hgmp.mrc.ac.uk Precedence: bulk I am a postdoc. working in non-wrom background Lab. Recently I identyfied and characterized a phophalipase homologe in C.elegans and it might also exist in germline because it was first found in testis. I meet a problem in doing in situ hybridization with whole mount C.elegans. most time I do the in situ , the result is not very consistant. To publish this paper soon, I am looking for some people who is skill at in situ hybridization techneque to cooperate with. Any one who would like to help will be highly apprecitaed and be a auther of this article. Please reply to: zhaobw@u.washington.edu Juan wrote in message news:8ecuiu$ff7$1@mercury.hgmp.mrc.ac.uk... > Hi, I am wondering if someone has performed RT-PCR on sigle worms using a > light cycler. If so, could you please provide with some tips/protocol. I > would eternally thank you for it. > > Juan > "Rougvie Lab" > --- --- .