From owner-drosophila@net.bio.net Sat Jun 03 23:00:00 1995
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From: kidd@rockyj.rockefeller.edu (Simon Kidd)
Subject: Re: orthophosphate labelling SL2 cells
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References: <1995Jun2.143128.29063@alw.nih.gov>
Date: Sun, 4 Jun 1995 17:10:58 GMT
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In article <1995Jun2.143128.29063@alw.nih.gov>, rchun@helix.nih.gov (Rene
Franz Chun) wrote:

> I'm looking into labeling a phosphoprotein in our drosophila
> SL2 cells.  I found a Gibco serum free media w/ met and cys
> omited which should work for our 35S labelings.  But didn't
> see a phosphate free medium?  
> 
> Thanks in advance for any suggestions!

You could always make up phosphate free M3 media and suppliment it with
dialysed serum. Worked for me.

.