From owner-celegans@net.bio.net Tue Jul 30 23:00:00 1996 Path: biosci!agate!garriga4.berkeley.edu!user From: forrestr@mendel.berkeley.edu (Wayne Forrester) Newsgroups: bionet.celegans Subject: cellular reporters Date: Wed, 31 Jul 1996 07:57:36 -0800 Organization: UCBerkeley Lines: 14 Message-ID: NNTP-Posting-Host: garriga4.berkeley.edu Hi, I am working on mutations in the C. elegans homeobox gene ceh-10, that appears to eliminate several cells. I would like to determine whether sister cells to those effected are present, and the easiest way to do this would be to use either GFP or lacZ reporters expressed in those cells, or by antibody staining to some factor expressed there. My question is, therefore, does anyone have any marker expressed in SMDDL/R, URxL/R, CEPsoDL, or OLLsoL/R. Any markers, staining techniques or any other method of identifying any of these cells would be immensely helpful. Thanks very much, Wayne Forrester (Garriga lab) forrestr@mendel.berkeley.edu .