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To: bionet-drosophila@moderators.isc.org
From: Chris Jones <cjones@moravian.edu>
Newsgroups: bionet.drosophila
Subject:  Re: Help with Lethal Mutations
Organization:  Moravian College Biology Dept.
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References:  <490D0AFAF3D2D3119F6C00508B6FDF1506965A9D@ex.mshri.on.ca>
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In article <490D0AFAF3D2D3119F6C00508B6FDF1506965A9D@ex.mshri.on.ca>,
 siddiqui@mshri.on.ca (Najeeb Siddiqui) wrote:

> I am new in working with Drosophila so this might be a naive question to
> some. I have received a piggyback (Exelexis) insertion line (balanced by
> TM6B, Tb[1] ) that is embryonic lethal for the insertion. I am wondering if
> there is an easy way to identify the dead embryos (due to homozygosity of
> the balancer) from the other dead embryos (dead due to homozygosity at the
> insertion locus)? I will greatly appreciate any help in this matter.

Put your insertion chromosome over another balancer with an 
easily-scored marker, such as GFP.

Chris Jones


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