From owner-biophysics@net.bio.net Sat Jan 31 22:00:00 1998
Path: biosci!agate!logbridge.uoregon.edu!europa.clark.net!208.134.241.18!newsfeed.internetmci.com!194.125.2.233!iol!easynet-uk!easynet-tele!peernews.ftech.net!news.ibm.net.il!ibm.net!news.biu.ac.il!not-for-mail
From: shahar stein <steins@inter.net.il>
Newsgroups: bionet.biophysics
Subject: Re: stray light
Date: Sun, 01 Feb 1998 10:15:00 +0200
Organization: Bar-Ilan University, Ramat-Gan, Israel
Lines: 17
Message-ID: <34D42F03.3EDD@inter.net.il>
References: <34D2370B.31A7@york.ac.uk>
NNTP-Posting-Host: ehrenbrg.ph.biu.ac.il
Mime-Version: 1.0
Content-Type: text/plain; charset=us-ascii
Content-Transfer-Encoding: 7bit
X-Mailer: Mozilla 3.01Gold (Win95; I)

David Scott wrote:
> Does anyone out there know anything about stray light in a fluorimeter
> and how to calculate/correct/get rid of it?
> We have an application where this may be a problem
> Thanks in advance

first of all it might be of help to read the manual, to see if they say
something about it and if not to call the supplyer. 
we have a perkin-elmer fluorimeter and one way is to minimize the
emission slit width.
 another option is to shut-off the lamp (try to measure by-lumini.)
and check the spectrum.

shahar stein
the biophysics lab
bar ilan university
isreal

.