From owner-diagnost@hgmp.mrc.ac.uk Wed Apr 27 10:00:49 2005 Return-Path: X-Original-To: diagnost-outgoing Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id 2319B519D2 for ; Wed, 27 Apr 2005 10:00:49 +0100 (BST) X-Original-To: diagnost-list@hgmp.mrc.ac.uk Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id DAFFF519D3 for ; Wed, 27 Apr 2005 10:00:48 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6045) id 0722E519D2; Wed, 27 Apr 2005 10:00:46 +0100 (BST) X-Original-To: bionet-diagnostics@net.bio.net Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id BADD47D11D for ; Fri, 31 Dec 2004 01:30:36 +0000 (GMT) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6022) id 87B457D0C3; Fri, 31 Dec 2004 01:30:32 +0000 (GMT) To: bionet-diagnostics@net.bio.net From: editor@gene-quantification.info (editor@gene-quantification.info) Newsgroups: sci.bio.misc,sci.med.diseases.hepatitis,bionet.diagnostics,sci.med.prostate.prostatitis Subject: qPCR Symposium Agenda NOW online Organization: http://groups.google.com Message-ID: X-Original-To: bionet-announce@net.bio.net Content-Type: text/plain; charset=ISO-8859-1 Content-Transfer-Encoding: 8bit X-Spamscanner: mailbox8.ucsd.edu (v1.5 Dec 3 2004 17:34:44, -2.3/5.0 3.0.0) X-MailScanner: PASSED (v1.2.8 19856 iBTLtsT8020124 mailbox8.ucsd.edu) Date: Wed, 27 Apr 2005 10:00:46 +0100 (BST) Sender: owner-diagnost@hgmp.mrc.ac.uk Precedence: bulk qPCR Satellite Symposium at the BioCity Leipzig Deutscher Platz 5, 04103 Leipzig, Germany http://leipzig05.gene-quantification.info/ organized by Dr. Michael W. Pfaffl and powered by http://www.BioScience-Events.com The main focus of the qPCR symposium is related to the key problems in real-time PCR. Optimisation, Normalisation & Standardisation Download the agenda http://www.bioscience-events.com/leipzig/agenda-qPCR-symposium.pdf Leading academic researchers and industrial contributors in the field will participate in the symposium, which will be an arena for fruitful discussions between researchers of different backgrounds. The Symposium offers an overview of the present knowledge and future developments in qPCR technology and its wide applications. The main focus of the talks will be on Optimisation, Normalisation and Standardisation of the real-time PCR and RT-PCR reaction. Starting from the pitfalls in tissue preparation, RNA or DNA extraction, we have a further focus on the cDNA synthesis (RT), and how to optimize the PCR reaction itself. Next key points in qPCR are the post qPCR working steps: how to determine the right Ct, which quantification strategy is the right one and how to calculate with the generated Ct data, BioInformatics (HKGs, normalisation...), to come to a biological meaningful results. Session 1: Pre-analytical steps (Moderator: M. W. Pfaffl) Session 2: Single cell PCR (Moderator: M. Kubista) Session 3: Probes Technologies (Moderator: S. Bustin) Session 4: Quantification strategies & Optimization (Moderator: S. Bustin) Session 5: Applications Moderator: (N. Zoric) Session 6: Post PCR & Bioinformatics (Moderator: M. W. Pfaffl) .