From owner-cellbiol@net.bio.net Sun Feb 02 22:00:00 1997
Path: biosci!agate!howland.erols.net!worldnet.att.net!news.maxwell.syr.edu!newsfeeds.sol.net!nntp.uio.no!usenet
From: Erik Kul| Hagen <e.k.hagen@labmed.uio.no>
Newsgroups: bionet.cellbiol
Subject: Transfection of B cells
Date: 3 Feb 1997 13:20:19 GMT
Organization: Universitet i Oslo
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We're trying to transient transfect different B cell-lines (Ramos, Raji, 
U698) using electroporation. We have tried different voltage, 
capacitance, with and without DEAE-dextran etc. with no satisfactory 
results. Does anyone have any experience in successfully transfecting any 
one of these above mentioned cell lines, if so I would be very pleased if 
you could reply.

Thank you for your attention!

Malfrid Roe
LIIPAT, The national hospital of Norway. 


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