From owner-biophysics@net.bio.net Thu Mar 05 22:00:00 1998
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From: fujimoto@u.washington.edu (Bryant Fujimoto)
Newsgroups: bionet.biophysics
Subject: Re: Lipari/Szabo calculations
Date: 6 Mar 1998 21:01:30 GMT
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Rasmus Storjohann <rstorjoh@sfu.ca> writes:

>I want to use relaxation matrix refinement and the Lipari-Szabo 
>model-free approach to improve my under-defined NMR structure. This means 
>that I need numbers for my correlation time and my order parameter. I was 
>planning to get my correlation time either from T1 relaxation at two 
>different field strengths, or from publinshed data based on Trp 
>fluorescence. (The correlation time from fluorescence is the correlatioin 
>time of overall motion, while the correlation time from T1 relaxation is 
>the correlation time of overall+internal motion, is that right?)

Any internal motion which the Trp undergoes will be reflected in its
correlation times.  Also, if the molecule is not spherical, then the
correlation times will depend on the orientation of the Trp or the
relative positions of the nuclei in the molecule.

Bryant Fujimoto
fujimoto@u.washington.edu


.