From owner-celegans@net.bio.net Wed Aug 07 23:00:00 1996 Path: biosci!agate!spool.mu.edu!news.sgi.com!news.msfc.nasa.gov!newsfeed.internetmci.com!in2.uu.net!news.Arizona.EDU!bonaire.ccit.arizona.edu!pmuhlrad From: Paul Muhlrad Newsgroups: bionet.celegans Subject: Re: Mailing frozen worms Date: Wed, 7 Aug 1996 17:15:30 -0700 Organization: The University of Arizona Lines: 19 Message-ID: References: <4uadet$gi2@news.uta.edu> NNTP-Posting-Host: bonaire.ccit.arizona.edu Mime-Version: 1.0 Content-Type: TEXT/PLAIN; charset=US-ASCII NNTP-Posting-User: pmuhlrad X-Sender: pmuhlrad@bonaire.ccit.arizona.edu In-Reply-To: <4uadet$gi2@news.uta.edu> I've sent worms successfully on dry ice many times. Along the same lines, but on another topic, our lab protocols and the Worm Book both say that when freezing worms it's important to let them approach -70 or -80 gradually by putting them inside a styrofoam container and putting that in the low temp freezer. In my experience, this is unnecessary. I routinely put fresh freezing stock in a non-insulated revco box in our -80. I've also just put the tubes, in the cryotube rack, straight in, and have not noticed any effect on recovery efficiency. For what it's worth, PJM ------------------------------------------------------------------------------ Paul Muhlrad University of Arizona, Department of Molecular and Cellular Biology ward_lab@tikal.biosci.arizona.edu (lab) | (520) 621-9135 phone pmuhlrad@u.arizona.edu (private) | (520) 621-3709 fax http://www.u.arizona.edu/~pmuhlrad .