From owner-biophysics@net.bio.net Fri Mar 25 22:00:00 1994
Path: biosci!agate!usenet.ins.cwru.edu!cleveland.Freenet.Edu!bl275
From: bl275@cleveland.Freenet.Edu (Dan Diaz)
Newsgroups: bionet.metabolic-reg,bionet.biophysics
Subject: Anion stimulation of enzymes - physiological or niet?
Date: 26 Mar 1994 05:14:46 GMT
Organization: Case Western Reserve University, Cleveland, OH (USA)
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Reply-To: bl275@cleveland.Freenet.Edu (Dan Diaz)
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Xref: biosci bionet.metabolic-reg:217 bionet.biophysics:144


i work with an e. coli exonuclease which is stimulated about ten-fold by 50
mM phosphate.  this is hardly physiological.  is the enzyme simply not as
active in vivo as in test tubo?  can other anions substitute in vivo?  any
suggestions for physiological anions to try in tubo?

.