From owner-celegans@net.bio.net Sat Nov 16 22:00:00 1996 Path: biosci!cb.uga.edu!ekipreos From: ekipreos@cb.uga.edu (Edward Kipreos) Newsgroups: bionet.celegans Subject: overexpression in C. elegans Date: 16 Nov 1996 21:23:01 -0800 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 24 Sender: daemon@net.bio.net Distribution: world Message-ID: NNTP-Posting-Host: net.bio.net I would like to have a means for obtaining overexpression from cDNAs. Does anyone have experience with promoters that could be used for constitutive or inducible overexpression in all cells. While the levels required for overexpression are going to be different between genes (as the normal expression levels vary), I would appreciate hearing of any experiences that people have had with overexpression from various promoters. Thanks, Edward --------------------------------- Edward T. Kipreos Department of Cellular Biology The University of Georgia 353 Biological Sciences Building Athens, GA 30602-2607 office: (706) 542-3862 lab: (706) 542-3137 fax: (706) 542-4271 email: ekipreos@cb.uga.edu --------------------------------- .