From owner-celegans@net.bio.net Wed May 20 23:00:00 1998 Path: biosci!biosci!not-for-mail From: Mike Palopoli Newsgroups: bionet.celegans Subject: inverted microscopes Date: 21 May 1998 06:43:54 -0700 Organization: BIOSCI International Newsgroups for Molecular Biology Lines: 56 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <6k1b2q$ds5@net.bio.net> NNTP-Posting-Host: net.bio.net To the C.elegans research community: A little over a week ago I posted a message on this list asking whether people had any recommendations for inverted microscopes, and also whether anyone had tried the new CARVer software from Atto Inc. I received two replies from members of the C. elegans community, both concerning inverted scopes -- Thank you for the advice. I also sent out a few direct inquiries, from which I received one reply. Below I reproduce the question and the relevant portions of the responses without disclosing the person responsible for the message (since I did not state in my original message that I would post responses to the list). >I am looking into the purchase of an inverted microscope that will be the >main workhorse for my new laboratory. I would like to get something that >will function effectively for microinjection and for general image capture >(brightfield, DIC, and fluorescence). Although I have not used a system >that is set up for 4D microscopy, the flexibility to use the system in this >way would be an obvious bonus. Do you have any recommendations? (1) We have had great luck with the Nikon Diaphot 200. We've done standard confocal, multiphoton, 4D DIC, laser ablation, optical tweezing, and Microinjection on ours. Never a problem. We got ours through the Fryer Company (1-708-669-2000). They're very helpful. (2) We've been happy with an Axiovert from Zeiss. But I understand that the new Nikon microscopes are excellent as well. And obviously, if you're thinking of 4D, you will neop of the line objectives. We've been happy with 15x eyepieces and 63x plan-apo. (3) The 2 main scopes I would recommend are the Olympus IX70 and the Leica DMIRB. Best regards, Mike. ____________________ Mike Palopoli, Ph.D. H.H.M.I., University of Chicago, MC1028, AMB N-101, 5841 S. Maryland Ave., Chicago, IL 60637 USA PH (lab): (773) 702-6274 PH (home):(773) 493-6393 FAX: (773) 702-0271 E-mail: palo@midway.uchicago.edu .