From owner-celegans@net.bio.net Mon Aug 18 23:00:00 1997 Path: biosci!agate!hammer.uoregon.edu!newsfeed.direct.ca!newsfeed.sovam.com!sovam!Gamma.RU!srcc!newsserv From: "Vladimir V. Bakaev" Newsgroups: bionet.celegans Subject: longevity in C. elegans Date: 19 Aug 1997 15:39:29 +0400 Organization: Private Person Lines: 42 Distribution: world Message-ID: Reply-To: vlad@bakaev.nsk.ru NNTP-Posting-Host: news.gamma.ru Mime-Version: 1.0 Content-Type: text/plain; charset=koi8-r Content-Transfer-Encoding: 8bit Summary: no positive effect Keywords: longevity, temperature, C. elegans X-Return-Path: bakaev!bakaev.nsk.ru!vlad@sinor.ru >From The Worm Breeder's Gazette, 1995, v. 14, No. 1, p. 102. THE EFFECT OF INTERMITTENT TEMPERATURE ON LONGEVITY AND FECUNDITY OF C. ELEGANS Vladimir V. Bakaev, box 45, Novosibirsk, 630107, USSR It is well known that low temperatures can prolong longevity of different animals. In this study the experimental worms were mantained in liquid medium with E. coli in +21oC during the day (8 - 12 hrs) and in +4oC during the night, in dark- ness. One control group was mantained in +21oC and other control group was mantained in +4oC constantly. The obtained results are presented in the following table. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Control group Experimental Control group (+21oC) group (+4oC) Mean+/-S.D. Mean+/-S.D. Mean+/-S.D. €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Mean longe- 19,86+/-1,63 22,96+/-1,57 38,3+/-2,72 vity (days) (n = 22) (n = 24) (n = 22) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Maximal lon- gevity (days) 34 35 50 €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Minimal lon- gevity (days) 6 10 5 €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Mean 76,91+/-4,54 54,33+/-3,32 4,54+/-2,07 fecundity (n = 22) (n = 24) (n = 22) €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Maximal 118 95 46 fecundity €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ Minimal fecundity 33 25 0 €€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€€ It can be concluded that such intermittent temperature is not able to prolong the life-span of C. elegans significantly, in comparison with constant cold, as well as fecundity. Acknowledgment: The author wishes to express his thanks to CGC for providing C. elegans (wild line) and E. coli OP50. .