From owner-biophysics@net.bio.net Thu Mar 24 22:00:00 1994
Newsgroups: bionet.biophysics
Path: biosci!lhc!nih-csl!Daniel.Geselowitz
From: dagesel@helix.nih.gov (Daniel Geselowitz)
Subject: Re: Isobestic point def'n wanted
Message-ID: <1994Mar25.181236.12644@alw.nih.gov>
X-Posted-From: InterNews 1.0@nih-csl.dcrt.nih.gov.
Lines: 8
Sender: -Not-Authenticated-[5085]
Organization: National Institutes of Health
References: <1994Mar23.143955.1@opal.tufts.edu> 
 <1994Mar23.230900.4807@alchemy.chem.utoronto.ca>  
 <Cn5w2F.L1q@info.bris.ac.uk>
Date: Fri, 25 Mar 1994 18:12:36 GMT
Xdisclaimer: No attempt was made to authenticate the sender's name.

Generally, an isosbestic point is a wavelength at which two different
substances have the same extinction (absorption) coefficient.  Thus,
during a reaction only converting one to the other, the absorbance at
this wavelength will not change.  The appearance of a good isosbestic
point during a reaction is usually an indication that the reaction
involves the net conversion of A to B.
                                     Daniel Geselowitz
                                     dagesel@helix.nih.gov

.