From owner-cellbiol@net.bio.net Wed Feb 19 22:00:00 1997 Newsgroups: bionet.molbio.methds-reagnts,bionet.cellbiol Path: biosci!rutgers.rutgers.edu!uwm.edu!news.cse.psu.edu!news3.cac.psu.edu!howland.erols.net!surfnet.nl!newsfeed.sunet.se!news99.sunet.se!news.ki.se!craciano From: Jarkko.Kortesmaa@mbb.ki.se (Jarkko Kortesmaa) Subject: Re: 293 cells in serum-free medium? Sender: news@news.ki.se (News admin) Message-ID: Date: Wed, 19 Feb 1997 13:20:53 GMT References: Nntp-Posting-Host: craciano.mbb.ki.se Organization: Karolinska Institute X-Newsreader: News Xpress 2.0 Beta #2 Lines: 15 Xref: biosci bionet.molbio.methds-reagnts:55075 bionet.cellbiol:6704 In article , graham@dbe.csiro.au (Lloyd Graham) wrote: >Does anybody know if 293 cells (Human Embryonic Kidney) tolerate >serum-free or reduced serum culture medium, and for how long? I'm hoping I have used the insulin-transferrin-selen supplement (Sigma) to replace serum for 293 cells. The cells do not like it too much but survive at least two days in it. There is minimal or no cell growth, though. Good luck,. ------------------------------------------------------------------------- Jarkko Kortesmaa Dept. of Matrix biology, MBB email: Jarkko.Kortesmaa@mbb.ki.se Karolinska Institutet Stockholm, Sweden ------------------------------------------------------------------------- .