From owner-cellbiol@net.bio.net Mon Dec 16 22:00:00 1996
Path: biosci!rutgers!uwm.edu!news-peer.gsl.net!news.gsl.net!EU.net!Ireland.EU.net!web3.tcd.ie!sci67033.sci.tcd.ie!user
From: brackena@tcd.ie (Adrian Bracken)
Newsgroups: bionet.cellbiol
Subject: X-linking proteins
Date: Tue, 17 Dec 1996 16:26:39 +0100
Organization: Trinity College, Dublin 2
Lines: 8
Message-ID: <brackena-1712961626390001@sci67033.sci.tcd.ie>
NNTP-Posting-Host: sci67033.sci.tcd.ie

Hi, I hope to add an enzyme which transiently interacts with its target
protein(s). I have antibodys for the enzyme and I have prepared a column
with these antibodies. I plan to run extracts through it. If I can somehow
link the enzyme to its target(s), prior to running on column, I should be
able to purify the enzymes targets??!! Has anybody got any practical tips
on how this has been done in the past? Has anybody done anything like
this? Thanks very much. Please could you forward mail directly to me at
brackena@tcd.ie.  Thanks a milion, Adrian Bracken.

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