From owner-dros@hgmp.mrc.ac.uk Sat Jul 13 23:30:49 2002 Return-Path: Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with SMTP id D251217A83 for ; Sat, 13 Jul 2002 23:30:48 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 8EE8617A8C for ; Sat, 13 Jul 2002 23:30:47 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 6020) id 07C5A17A83; Sat, 13 Jul 2002 23:30:45 +0100 (BST) Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 0210317BAE for ; Sat, 13 Jul 2002 18:21:08 +0100 (BST) Received: by mercury.hgmp.mrc.ac.uk (Postfix, from userid 60001) id ECC3117BA8; Sat, 13 Jul 2002 18:21:06 +0100 (BST) To: bionet-drosophila@net.bio.net From: mitch.dushay@sh.se (mitch dushay) Newsgroups: bionet.drosophila Subject: P element transpositision distance/frequency Organization: BIOSCI/MRC Human Genome Mapping Project Resource Centre Message-ID: X-Received: from localhost (localhost [127.0.0.1]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 3F36517BAA for ; Sat, 13 Jul 2002 18:21:04 +0100 (BST) X-Received: from grace.grace.se (grace.grace.se [193.14.11.2]) by mercury.hgmp.mrc.ac.uk (Postfix) with ESMTP id 6B63E17BA8 for ; Sat, 13 Jul 2002 18:21:02 +0100 (BST) X-Received: from [193.11.41.203] (root@localhost) by grace.grace.se (980427.SGI.8.8.8/980728.SGI.AUTOCF) via SMTP id TAA14213 for ; Sat, 13 Jul 2002 19:19:01 +0100 (CET) X-BlackMail: 193.11.41.203, [193.11.41.203], , 193.11.41.203 X-Authenticated-Timestamp: 19:19:11(CET) on July 13, 2002 X-Sender: shmhdy@eden.grace.se X-To: dros@net.bio.net Date: Sat, 13 Jul 2002 23:30:45 +0100 (BST) X-Razor-id: 2f2774ff2c07cae8b5f3e835eb2a6d6f1a264665 Sender: owner-dros@hgmp.mrc.ac.uk Precedence: bulk Dear Fly Community members, We're trying to hit a small gene with an enhancer trap P element, and it would be useful to know more about the frequency of P transpositions to different distances. We've studied the Zhang and Spradling, and Tower et al. papers from 1993, and we wonder if anyone has more data on what percentage of P transpositions remain on the same chromosome arm, and what percent are withiin 150, 100, 50 ... 2 Kb, etc. from dysgenic males or females. We would be grateful for any additional data on this issue. I'll be happy to discuss our specific details by private email - I hate to waste public bandwidth. Thanks! Mitch Mitchell Dushay Avdelningen för naturvetenskap Section för insekt och medfödd immunitet Södertörns högskola phone 46 8 608 47 43 141 89 Huddinge Sweden FAX: 46 8 608 45 10 --- .