From owner-drosophila@net.bio.net Wed Oct 04 23:00:00 1995
Path: biosci!BIOMED.UAMS.EDU!hbenes
From: hbenes@BIOMED.UAMS.EDU (Helen Benes)
Newsgroups: bionet.drosophila
Subject: Any Delta 2-3 on 2nd Chromosome
Date: 5 Oct 1995 10:41:06 -0700
Organization: BIOSCI International Newsgroups for Molecular Biology
Lines: 22
Sender: daemon@net.bio.net
Distribution: world
Message-ID: <3073DCE4@mail.uams.edu>


I sent this message last week, and haven't heard from anyone.  Is it 
possible that there no "Delta 2-3 on the second chromosome" stock is 
available?  Or is my message not reaching the Drosophila community?  Could 
someone please just respond so that I know that I am being read?  Thanks!

In my lab we are trying to apply P element local hopping to mutagenesis of 
the Drosophila Lsp-2 gene at 68E3,4.  We are starting with a P[w+] element 
at 68E1 and so far (2000 F2 males screened by PCR) have no success.  We 
estimate that the original element is about 135 kb from our target.  We are 
using as a source of transposase, a Delta 2-3 on the third chromosome, a TM3 
Sb.  As we consider altering our strategy, we're looking for a source of P 
element transposase on the first or second chromosome.  If anyone has such 
stocks, please contact me.  Also if anyone (out there) is willing to share 
their favorite local hopping protocol and experience (e.g., distance between 
original P element and target) with us, we would be most grateful.

Helen Benes
Department of Biochemistry/Molecular Biology
University of Arkansas for Medical Sciences, Slot 516
4301 West Markham St.
Little Rock, AR 72205

.