From owner-celegans@net.bio.net Tue Jan 09 22:00:00 1996
Path: biosci!bcm.tmc.edu!news.msfc.nasa.gov!newsfeed.internetmci.com!tank.news.pipex.net!pipex!peer-news.britain.eu.net!lyra.csx.cam.ac.uk!NewsWatcher!user
From: tamsin@zoo.cam.ac.uk (Tamsin Braisher)
Newsgroups: bionet.celegans
Subject: Sonication of nematode eggs
Date: 10 Jan 1996 21:23:09 GMT
Organization: Cambridge University
Lines: 16
Message-ID: <tamsin-1001962129040001@131.111.46.107>
NNTP-Posting-Host: wa-mac5.gen.cam.ac.uk

I am attempting to extract DNA from trichostrongylid eggs using the
protocol given in Gasser et al (1993). This uses ultrasonication (at 12um
for 30 seconds). My problem is that the only sonicator available to me
works on a 1-100% basis, with no manual available. I don't know how this
relates to micrometers - I have contacted the company (Misonix) and
received no reply - can anybody help? If anyone has any experience of
using a Misonix W-375 sonicator with cup-horn attachment, I would be very
grateful for any help!

Thanks,

Tamsin Braisher
Department of Genetics
University of Cambridge

tamsin@zoo.cam.ac.uk

.