From owner-celegans@net.bio.net Mon Aug 16 12:54:00 1999 Path: biosci!biosci!not-for-mail From: gidi Newsgroups: bionet.celegans Subject: Re: dsRNA Soaking Date: 16 Aug 1999 06:54:08 -0700 Organization: NetVision Israel Lines: 32 Sender: daemon@net.bio.net Approved: wmorgan@acs.wooster.edu Distribution: world Message-ID: <7p9560$n68@net.bio.net> NNTP-Posting-Host: net.bio.net Hi chris. My name is gidi shemer and I'm also after my first year in the phd (I'm working in the technion, israel in beni podbilewicz lab). I have'nt tryed the soaking method but if you want some details, I thing you should write to Ron Eliss. In the last meeting he showed some nice staff about this method with the gene fem-1. As for the positive control, you can use apx-1. You should get dead embryos arested at about 50 cells stage with hyper pharyngal cells. If you want more details, e.mail me at work (bishemer@tx.technion.ac.il). bye, gidi. "CHRIS.J.MEE" wrote: > As a first year PhD student who is currently working with dsRNA > interference in C.elegans i was interested to see in some of the > abstracts at the 1999 International Worm Meeting that a number of > people were using dsRNA soaking as a method of RNAi instead of > microinjection. > I dont seem to be able to find anything further on the technique so i > was hopeing that someone may be able to fill me in on how its done > and how successful it is compared to microinjection of dsRNA. > I would also like to know if anybody has a good gene to use as a > positive control in dsRNA injections as the genes i am looking at > have not been used before and so i have no idea if they give an > obvious phenotype. It may be that my injection technique needs work > so a positive control with a good phenotype would help. > > Any help would be great > Chris Mee > University of Nottingham > plxcjm@nottingham.ac.uk > --- .