From owner-drosophila@net.bio.net Mon Mar 04 22:00:00 1996 Path: biosci!bcm.tmc.edu!usenet From: Andrea Marie Peier Newsgroups: bionet.drosophila Subject: EMS mutagenesis Date: Mon, 04 Mar 1996 18:39:39 -0600 Organization: Baylor College of Medicine, Houston, Tx Lines: 9 Message-ID: <313B8D4B.41C67EA6@bcm.tmc.edu> NNTP-Posting-Host: kiwi.imgen.bcm.tmc.edu Mime-Version: 1.0 Content-Type: text/plain; charset=us-ascii Content-Transfer-Encoding: 7bit NNTP-Posting-User: ap691301 X-Mailer: Mozilla 2.0 (X11; I; SunOS 4.1.3_U1 sun4m) Why does sperm storage (treated with alkylating agents such as EMS) in females effect the types of mutations recovered. Ashburner states that when sperm treated with EMS are stored in the female reproductive tract, there is an increase in the number of chromosome aberrations recovered vs. point mutations. I'm not exactly clear why . Can someone explain it to me? Best regards, Andrea .