From owner-celegans@net.bio.net Mon May 12 23:00:00 1997
Path: biosci!MEDEW.NEMA.WAU.NL!qin.ling
From: qin.ling@MEDEW.NEMA.WAU.NL (Qin Ling)
Newsgroups: bionet.celegans
Subject: male transformation by microinjection?!
Date: 13 May 1997 03:01:13 -0700
Organization: Agricultural University Wageningen, Virology
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Sender: daemon@net.bio.net
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Message-ID: <01IITJL5B0WS00CUG9@NET.WAU.NL>
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Helllo, everybody out there!

We are not C. elegans people. But we are excited by the development in C. 
elegans field. So we would like to borrow some new tricks you guys did to 
C. elegans and try to use them on plant parasitic nematodes we are 
interested in. First we want to microinject DNA into the female gonad to 
transform them. But these females have thick cuticles and they are not 
transparent at all! So we are now thinking to transform the males and let 
the males do the job instead. But we are not sure whether after injecting 
DNA into male gonad, we will end up with transformed progenies.

 So help wanted!!!
 This may sound silly! BUT:
1. Anybody ever tried microinjection in males of C. elegans?
2. Can you see the male gonad as clear as hermaphrodite gonad?
3. If I can put DNA into male gonad, do you think the foreign DNA will go 
together with sperms to fertilize the oocytes? Will the cytoplasm volume 
of oocyte or sperm decide the chance of trnaformation? Will the foreign 
DNA interact with all those nucleus in male gonad, hence they 
get incorporated into sperms?

Your suggestion will be highly appreciated!
HELP WANTED!
Thank you!



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