From owner-drosophila@net.bio.net Thu Jul 06 23:00:00 1995 Path: biosci!rutgers!uwm.edu!fnnews.fnal.gov!unixhub!news.Stanford.EDU!not-for-mail From: adhar@leland.Stanford.EDU (Adam Noel Harris) Newsgroups: bionet.drosophila Subject: Re: NEEDLES Date: 7 Jul 1995 10:53:23 -0700 Organization: Stanford University Lines: 10 Distribution: world Message-ID: <3tjsaj$76p@elaine42.Stanford.EDU> References: <29686003447@osmosis.biology.emory.edu> NNTP-Posting-Host: elaine42.stanford.edu X-Newsreader: TIN [version 1.2 PL2] Victoria Finnerty (victoria@OSMOSIS.BIOLOGY.EMORY.EDU) said this stuff: : DOES ANYONE HAVE INFORMATION ON SHARPENING NEEDLES FOR : TRANSFORMATION, PERHAPS BY USING A FINE ABRASIVE IN : AN OIL BATH ??? Needles pulled from glass capillary tubes have been adequately sharp in my experience. You may want to look into purchasing such a puller. -Adam .